VOLUMETRIC IMAGING,
AI BASED, FULLY AUTOMATED CELL COUNTING AND CLASSIFICATION
Meet us at AlgaEurope 2024 in Athens from December 10 to the 13th! We will be showcasing the latest enhancements to our Holodetect microscope product family. Holodetect HiRes + Fluor++
now features a multiple wave-length fluorescent microscope capable of capturing emissions from chlorophyll, phycocyanin/phycoerythrin, respectively. We will also be introducing our newly developed multispectral biomass detector, a high-accuracy, cost-effective solution for inline monitoring of your culture's biomass development. We look forward to meeting potential partners from the microalgae industry and research.
Holodetect sensors and instruments utilize Digital Holographic Microscopy (DHM) and artificial intelligence to automatically analyze volumetric liquid samples to detect, classify, and count objects, cells, and microorganisms in the 3-400 micron range.
Holodetect DHMs are well suited for inline, automated cell count measurements by class. In monocultures, Holodetect can distinguish algae cells from other objects in the sample (debris, small objects, etc.). In multi-species cultures, it provides cell count by species. It also gives an accurate estimate of cell count in cell conglomerates. It measures detailed cell morphology (cell size, shape parameters). Using the built-in fluorescent microscope, a measure of autofluorescence and an estimate of chlorophyll (or other auto-fluorescent molecule) content can be obtained. With appropriate labeling, living and dead cells can be counted separately.
Holodetect enables rapid, AI-enhanced, fully automated cell counting and classification.
Holodetect devices are automatic volumetric microscopes featuring flow-through measurement cuvettes with depths ranging from 100 to 10,000 microns to accommodate different magnification needs. Leveraging the power of digital holographic microscopy, Holodetect captures the entire sample depth in a single image, eliminating the need for optical refocusing as it is done numerically post-recording. This technology allows for reconstructing all sample objects from the recorded hologram with minimal sample preparation.
The analysis of reconstructed images is powered by advanced artificial intelligence. These AI models are highly adaptable and can be trained to identify various species and objects within the sample accurately. Additionally, our holographic microscope can be integrated with fluorescent microscopy to enhance classification precision and conduct a variety of fluorescence-based measurements. This synergy between technologies ensures comprehensive sample analysis with unparalleled accuracy.
Holodetect Instruments Ltd. is a spin-off company of HUN-REN SZTAKI, a research institute with the fundamental task of performing basic and application-oriented research in an interdisciplinary setting in the fields of computer science, engineering, information technology, intelligent systems, process control, wide-area networking and multimedia.
Holodetect devices are developed in collaboration with the Computational Optical Sensing and Processing Laboratory of HUN-REN SZTAKI. Our team’s main R&D focus is the development of digital holographic and fluorescent microscopes with applications, among others, in water utilities, microalgae, and beer brewery industries.
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