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VOLUMETRIC IMAGING,
AI BASED, FULLY AUTOMATED CELL COUNTING AND CLASSIFICATION

NEWS

Meet us at AlgaEurope 2024 in Athens from December 10 to the 13th! We will be showcasing the latest enhancements to our Holodetect microscope product family. Holodetect HiRes + Fluor++
now features a multiple wave-length fluorescent microscope capable of capturing emissions from chlorophyll, phycocyanin/phycoerythrin, respectively. We will also be introducing our newly developed multispectral biomass detector, a high-accuracy, cost-effective solution for inline monitoring of your culture's biomass development. We look forward to meeting potential partners from the microalgae industry and research.

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PRODUCTS

Holodetect sensors and instruments utilize Digital Holographic Microscopy (DHM) and artificial intelligence to automatically analyze volumetric liquid samples to detect, classify, and count objects, cells, and microorganisms in the 3-400 micron range.

Holodetect DHMs are well suited for inline, automated cell count measurements by class. In monocultures, Holodetect can distinguish algae cells from other objects in the sample (debris, small objects, etc.). In multi-species cultures, it provides cell count by species. It also gives an accurate estimate of cell count in cell conglomerates. It measures detailed cell morphology (cell size, shape parameters). Using the built-in fluorescent microscope, a measure of autofluorescence and an estimate of chlorophyll (or other auto-fluorescent molecule) content can be obtained. With appropriate labeling, living and dead cells can be counted separately. 

WHAT WE DO

Holodetect enables rapid, AI-enhanced, fully automated cell counting and classification.

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AUTOMATED CELL COUNTING

Holodetect offers minimal sample preparation, without the need for specialized personnel. It operates in flow-through mode, allowing analysis of larger sample volumes while maintaining validated counting accuracy. The system measures the morphological characteristics of different cell types and their distribution, providing accurate count estimates even for cells in large conglomerates. 
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FLUORESCENCE MEASUREMENTS

Our HiRes+Fluor system combines an integrated fluorescence microscope with automatic cell counting based on fluorescence. It provides precise fluorescence intensity measurements at both the cell and sample level, allowing accurate estimation of chlorophyll content. The system also distinguishes between living and dead cells and supports labelled measurements using species- or function-specific dyes.

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AI BASED CLASSIFICATION

The AI-based system offers advanced object detection, segmentation, and classification. With flexible retraining capabilities, it can classify and count different species, including multi-species cultures. The few-shot learning module allows for rapid on-site retraining, ensuring quick adaptation to new species or conditions.

DIGITAL HOLOGRAPHY

Holodetect devices are automatic volumetric microscopes featuring flow-through measurement cuvettes with depths ranging from 100 to 10,000 microns to accommodate different magnification needs. Leveraging the power of digital holographic microscopy, Holodetect captures the entire sample depth in a single image, eliminating the need for optical refocusing as it is done numerically post-recording. This technology allows for reconstructing all sample objects from the recorded hologram with minimal sample preparation. 

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ADVANCED FEATURES

The analysis of reconstructed images is powered by advanced artificial intelligence. These AI models are highly adaptable and can be trained to identify various species and objects within the sample accurately. Additionally, our holographic microscope can be integrated with fluorescent microscopy to enhance classification precision and conduct a variety of fluorescence-based measurements. This synergy between technologies ensures comprehensive sample analysis with unparalleled accuracy. 

DIGITAL OBJECT FOCUSING

Once the hologram has been recorded, it can be digitally reconstructed at different depths. The following examples show how the object comes into focus when the hologram is propagated at different depths. This allows us to efficiently capture an entire volume of space and quickly and accurately identify objects at different depths.
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Nematode
Large Volume DHM
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Rotifer
Large Volume DHM
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Spirulina
Large Volume DHM
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Haematococcus
High Resolution DHM
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Scenedesmus
High Resolution DHM
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Yeast
High Resolution DHM

SOLUTIONS & SERVICES

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INDUSTRIAL SOLUTIONS AND INTEGRATION

Holodetect instruments can be interfaced with existing or under development industrial production processes (e.g. food processing, microalgae culturing, water quality monitoring). We are ready to implement custom sampling and measurement data relaying to the control loop in custom systems.

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AI MODEL TRAINING AND TRAINING DATABASE ANNOTATION

The AI models built in Holodetect can be flexibly trained and retrained to classify new species or objects in fluids. We would be happy to assist you in AI model design and training. In order to successfully train deep learning models, a significant amount of annotated training data is required. We can help you create these databases.
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ABOUT US

Holodetect Instruments Ltd. is a spin-off company of HUN-REN SZTAKI, a research institute with the fundamental task of performing basic and application-oriented research in an interdisciplinary setting in the fields of computer science, engineering, information technology, intelligent systems, process control, wide-area networking and multimedia.

Holodetect devices are developed in collaboration with the Computational Optical Sensing and Processing Laboratory of HUN-REN SZTAKI. Our team’s main R&D focus is the development of digital holographic and fluorescent microscopes with applications, among others, in water utilities, microalgae, and beer brewery industries.

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