VOLUMETRIC IMAGING,
AI BASED, FULLY AUTOMATED CELL COUNTING AND CLASSIFICATION
Holodetect enables rapid, AI-enhanced, fully automated cell counting and classification.
Holodetect sensors and instruments utilize Digital Holographic Microscopy (DHM) and artificial intelligence to automatically analyze volumetric liquid samples, detecting, classifying, and counting objects, cells, and microorganisms in the 3-400 micron range.
Holodetect DHMs are well-suited for inline, automated cell count measurements by predefined classes. In monocultures, Holodetect can distinguish cells from other objects in the sample (such as debris or small particles). In multi-species cultures, it provides cell counts by species. It also gives an accurate estimate of cell counts in cell conglomerates and measures detailed cell morphology, including cell size and shape parameters. Using the built-in fluorescence microscope, it can measure autofluorescence and estimate chlorophyll (or other autofluorescent molecule) content. With appropriate labeling, living and dead cells can be counted separately.
Holodetect Instruments will showcase its latest developments at AlgaEurope 2025 in Riga. Among other novelties, a new, versatile low-cost inline digital holographic microscope, and new sampler/diluter that connects to multiple reactors and doubles as an inline turbidity and biomass sensor will make their debut. Visit us at stand 16 between December 9-12 in Riga!
Holodetect devices are automatic volumetric microscopes featuring flow-through measurement cuvettes with depths ranging from 100 to 10,000 microns to accommodate different magnification needs. Leveraging the power of digital holographic microscopy, Holodetect captures the entire sample depth in a single image, eliminating the need for optical refocusing as it is done numerically post-recording. This technology allows for reconstructing all sample objects from the recorded hologram with minimal sample preparation.
The analysis of reconstructed images is powered by advanced artificial intelligence. These AI models are highly adaptable and can be trained to identify various species and objects within the sample accurately. Additionally, our holographic microscope can be integrated with fluorescent microscopy to enhance classification precision and conduct a variety of fluorescence-based measurements. This synergy between technologies ensures comprehensive sample analysis with unparalleled accuracy.
Holodetect Instruments is a technology-driven company specializing in advanced inline monitoring solutions for algae cultivation and bioprocessing. We develop and manufacture digital holographic and fluorescence microscopes combined with AI-enabled analytics to help operators see and act on their processes in real-time. Our systems deliver continuous, label-free imaging of microorganisms in flow, with optional fluorescence channels for species-specific detection. Using our Quick AI Trainer (QAIT), customers can rapidly adapt models to their own strains and processes, ensuring high accuracy in diverse conditions. Holodetect’s instruments enable higher productivity, fewer contamination losses, and optimized resource use in photobioreactors, fermenters, and water utilities.
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